Immuno Oncology Biomarker Analysis
                     ®
On-Demand Digital Pathology OptraScan®
Affordable, Subscription-based System
Programmed Death-Ligand (PD-L1) Automated Image Analysis
Programmed Death-Ligand 1 expression is an 
important cancer biomarker and has become a 
cr i t ical  part  of  pat ient management for 
immunotherapy. The targeted blockade of 
PD-L1 may help to restore the antitumor 
response. Development of the combined positive 
score (CPS) for the evaluation of PD-L1 in solid 
tumors has indicated that PD-L1 expression on 
both tumor and tumor-associated immune cells 
is associated with clinical outcome. 
OptraSCAN's Solution for Immuno-oncology
Optra PD-L1 automated image analysis for research use, supports interpretation of PD-L1 IHC expression on both tumor and 
immune cells in solid tumors. It determines PD-L1 expression on tumor and immune cells showing partial or complete 
membrane staining on tumor cells alongwith membrane and/or cytoplasmic staining on immune cells in a single read. It is 
reliable and can be used to assess PD- L1 expression in a more objective manner.
General considerations by the PD-L1 assay
Ø Fully automated algorithm evaluates and generates scores for both, regions of interest and whole slide analysis with 
minimal human intervention
Ø Score generation based on the number of PD-L1 positive cells (tumor, lymphocytes and macrophages) in relation to total 
tumor cells allows capturing of tumor and immune cells in a single read
Ø Fully integrated image acquisition and automated evaluation of tumor area using Computer aided region detection system 
based on Artificial Neural Network
Ø Reporting module included with case management
The following analytical components are assessed by the AI tool
m Tumor Cell staining (an estimate of the percentage of tumor cells exhibiting partial or complete membranous staining)
m Immune Cell (IC) staining (an estimate of the percentage of ICs, including macrophages and lymphocytes, within the 
tumor, exhibiting staining). 
INPUT OUTPUT INPUT OUTPUT INPUT OUTPUT
High Expression                No Expression                  Low Expression
Posive cells Macrophages Immune cells Stained Membrane Negave cell
OptraScan® ®On-Demand Digital Pathology  Solutions 
OS-15 OS-120 OS-FS OS-FL
15-slide brightfield 120-slide brightfield 7-slide frozen sections, 15-slide fluorescence, 
with live view mode with 6 filter cubes 
IMAGEPath®
Web-based Image Management and Viewing
TELEPathTM OptraASSAYS
TM
Web and Mobile Digital Conferencing On-Demand Image Analysis
CLOUDPath®
Laboratory Information Management System
100 Century Center Court,
Suite 410, San Jose, CA 95112
OptraSCAN is an ISO13485 certified company *All OptraSCAN systems and solutions are for research use only