Automatic Gradient thermal cycler has a sophisticated operating computer controlled software to guide the protocols for PCR analysis for once and it can operate the functions offline. The high-precision Peltier heating units provides maximum. heating rates within the range of 0 to 100 °C. It is the suitable solutions to fulfill different requirements like adding samples while process is going on to encounter several experimental requirements. We have optimized capacity of 96 wells plate and half skirt 96 wells plate. It supported by user-friendly USB port or RS232 / RS485 port to connect with computer and print data.