Gradient thermal cycler enabled with Peltier heating units which can process more than 100 cycles and store data in the internal flash memory. It can accommodate 96×0.2 ml sample in wells. User-friendly programming is achieved via 8-inches TFT color touch-screen with graphical display and windows operations system. This device can be applied across a temperature range of 0 to 100 °C with maximum. ramping rate is 4.5 °C/s. It is advantageous to use pre-installed 11 standard protocols and secure his data using password Login method. The files can be transferred via USB, printer support.