Top Press Freeze dryer machine offers the ability to stabilize and preserve numerous biologicals in laboratory research and development. The unit provides a real time display of collector temperature, vacuum level and point detection system. The unit has a freeze drying area of 0.09 m2 with a total loading capacity of 0.9L. For the freeze drying process, the refrigerants R600a + R290 + R23 were employed, which are fluorine-free refrigerants. In laboratory study, they have the ability to maintain and preserve a wide range of biologicals.