Swine kidney fibroblasts are reliable in vitro tools for renal research owing to the similarities between pigs and humans. Fibroblasts are essential for maintaining the renal structural framework by producing the extracellular matrix. Due to their role in repairing tissue injury and fibrosis, they have become a focal point of research. Therefore, research on swine kidney fibroblasts has focused on studying kidney functions, understanding renal disease mechanisms, and formulating innovative treatments.