Understanding Cell Lines in Cancer Research
                     UNDERSTANDING CELL 
LINES in
Cancer
Research
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What are Cancer 
Cell Lines
Cancer cell lines: Cells derived from cancerous 
tissue cultured and maintained in lab conditions 
for continuous growth
Cells used for research (various study fields: Cancer 
biology, drug testing, Cytotoxicity study, Cancer 
Therapeutics)
Cell lines mimic the cancer 
microenvironment and properties
Enable investigation of compounds or drugs for 
cancer treatments in a  controlled environment.
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Primary Cells Versus Cell 
Lines
Primary
Derived directly from living tissue Cells that are cultured for extended time 
(in- vivo culture) periods (in vitro culture)
representative  of  cells  in  the  Capable of indefinite growth
human body Not as representativeof natural
Limited   lifespan   and   requires biological systems
  a controlled environment and Convenient for large-scale or
medium for survival epxrepleimrimineanry
Best for studying the real ts
physiological responses of a  drug Used  for  various  studies  in  animal  cell  
culture. Example:  Drug  screening,  
Cytotoxicity  Testing and Cellular and 
Molecular Studies
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Immortalised Cell 
LinImemsortalised Cell Lines are primary cells 
that are genetically modified to 
proliferate indefinitely.
Achieved by introducing oncogenes or 
other factors that can skip normal 
cellular senescence.
Cells can be used for long-term 
studies Easy, consistent experimental 
conditions Commonly used in drug 
development and genetic research
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Applications
Drug Development: Testing drugs
Genetic Studies: Gene expression and 
Cancer mutations
Cancer Biology: Studying cancer 
progression, metastasis, angiogenesis, 
resistance mechanisms, etc.
Immunotherapy: Testing the 
effectiveness of immunotherapy drugs 
on cancer cell lines.
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Conclusion & Future 
Directions
Innovations: Advancements in 
technology enable the use of realistic 
models like 3D cell culture systems and 
organoids
Challenges: Cell lines do not replicate 
the exact physiological conditions of 
the tumour
Conclusion: Cancer cell lines are 
invaluable in research, enabling the 
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models; testing of new drugs
Thank 
You
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