Radial Immunodiffusion Testing Service
                     Creative Diagnostics Announces the Launch of Radial Immunodiffusion Testing  
Service for Researchers
As an expert in providing solutions to assist virology and microbiology research,  
Creative Diagnostics has launched its new Single Radial Immunodiffusion Testing  
services for the biological industry that facilitate researchers in determining the  
amount or concentration of antigens in a sample.
Single radial immunodiffusion (SRID) is an agar gel precipitation test used to  
determine the amount or concentration of antigens in a sample. Antibodies of  
known specificity are uniformly distributed in an agar gel, and a sample containing  
the antigen of interest is placed in a well in the gel. The antigen then diffuses radially  
from the well to form a precipitate ring at the point of antibody-antigen equilibrium.  
Finally, the concentration of the antigen is determined by measuring the diameter of  
the precipitate ring and by extrapolation using a standard curve.
WHO guidelines require vaccine manufacturers to determine the potency of their  
products at the time of release and throughout the licensed shelf life. The SRID assay  
is the current gold standard for influenza vaccine potency. The assay is based on the  
diffusion of viral antigen into an agarose gel containing antibodies specific for the  
antigen being tested. Meanwhile, the interaction between antigen and antibody  
produces a precipitation zone whose size is proportional to the amount of antigen  
applied. Thus, the potency value of an unknown preparation can be determined by  
comparing the size of the precipitation zone produced by the unknown preparation  
to the sizes of zones obtained with a calibrated reference of known antigen content.
Creative Diagnostics’ new SRID testing services provide customers with accurate and  
reliable results for their samples. In addition to immunology, i.e., determining the  
amount or concentration of antigen in a sample, these services can be used to  
estimate immunoglobulin classes and IgG and IgM antibodies in serum, determine  
the relative purity of an antigen preparation, and perform serologic studies.
The SRID testing service can provide customers with easily interpretable results, and  
its sensitivity and specificity depend on the quality of the antibodies used in the  
assay. Because SRID has been in use for decades, there is a large amount of historical  
data and experience. It measures the antigens considered most relevant to  
protection, especially those in the original conformation. It can also be used to test  
the potency of the final product, which is almost always polyvalent.
Creative Diagnostics’ experienced scientists can provide customers with expert  
advice on how to optimize their assays for maximum sensitivity and specificity. The  
company’s state-of-the-art equipment and techniques ensure that all samples are  
tested to the highest standards.
For more information about Creative Diagnostics’ Single Radial Immunodiffusion  
Testing Service, please visit
https://antiviral.creative-diagnostics.com/single-radial-immunodiffusion-srid-testing-  
service.html.
About Creative Diagnostics
Headquartered in New York, Creative Diagnostics is a consulting and experimental  
service provider specializing in virology and microbiology. The company provides  
comprehensive solutions to conquer obstacles in virology and microbiology research,  
from high-security infrastructure provision, biosafety regulation elucidation, to  
expert viral system assistance.