Uploaded on Apr 27, 2023
PPT on Colorimetry
Colorimetry
COLORIMETRY
INTRODUCTION
Colorimetry has been around since the 1870s when
Jules Dubosq invented the colorimeter.
In 1885 Joseph William Lovibond devised a method of
measuring colors with a handheld comparator while
working in his father's brewery.
A TECHNIQUE
Colorimetry is a technique for measuring the
concentration of a known constituent in a solution
compared to standard solutions.
ABOUT COLORIMETRY
The human eye perceives light and color in a very
subjective way. Science demands an objective way of
measuring things, which is colorimetry.
Mathematically, the Beer-Lambert Law, which is a
combination of two laws, defines colorimetry.
BEER-LAMBERT LAW
Beers Law says that if monochromatic light passes through
a homogenous solution, the amount of light transmitted is
directly proportional to the solute in the solution.
Lamberts Law states that the length of the path
determines the absorbance of light by a colored solution
and the volume of liquid it passes through
COLORIMETRY USES BEER-LAMBERT LAW
Colorimetry uses this law to measure the absorbance
(more frequently) or light transmittance to determine
solution concentrations.
The solution must not fluoresce, and there should be no
temperature change during the measuring process.
COLOR COMPARATORS AND COLORIMETERS
The principle of Lovibond's comparator is still in use
today, and indeed the "Lovibond Comparator" is still a
brand of the Tintometer company in the UK.
This type of colorimeter, sometimes called a "Filter
Photometer," consists of a handheld cube with space for
two cuvettes into which liquids for analysis are placed.
AN ILLUMINANT
A specific light source (daylight in a comparator),
usually an LED or incandescent light, which projects a
fixed, consistent light onto an object.
AN OBSERVER
A specified field of view to analyze colors. A standard
observer is usually two degrees in a colorimeter
USES OF COLORIMETRY
Colorimetry is used in monitoring to measure bacterial
growth rates, quality, and color in food and beverage
manufacturing, textiles manufacturing, cosmetics, paint
works, and the petroleum industry.
THANK YOU
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