What is meant by micromanipulation?
                     WHAT IS MEANT BY 
C
MICROMANIPULATION?
INTRODUCTION
• Micromanipulation is a technique used in 
assisted reproductive technology (ART) that 
involves the manipulation of the eggs, 
sperm, and embryos at a cellular level using 
microscopic instruments by an embryologist.
Source: www.fertilitysmarts.com
What is the use?
• It can be used to inject a sperm into a 
mature egg to aid fertilization, to assist with 
the hatching of a blastocyst, and for the 
diagnosis of genetic abnormalities in the 
embryo prior to implantation.
Source: www.fertilitysmarts.com
Increase the chances of a 
successful pregnancy 
• Micromanipulation can help individuals or 
couples with both male and female factor 
infertility to increase the chances of a 
successful pregnancy through in vitro 
fertilization (IVF).
Source: www.fertilitysmarts.com
How it works?
• Micromanipulation techniques use expensive and 
require delicate instruments smaller than 
embryo cells that are connected to fine motor-
driven robot arms controlled by complex 
joysticks and pedals. 
• Sometimes microscopic laser beams are used to 
make openings or cuts in cell membranes.
Source: www.fertilitysmarts.com
Microsurgery
• Due to the level of sophistication of this process, 
micromanipulation is also called cell surgery or 
microsurgery. 
• Micromanipulation is often used in procedures 
that are designed to "workaround" either 
existing or unknown conditions that may impact 
fertility.
Source: www.fertilitysmarts.com
Intracytoplasmic sperm 
injection (ICSI)
• An individual sperm is collected and injected into 
the cytoplasm of a mature egg
Source: www.fertilitysmarts.com
Assisted hatching (AZH)
• The outer protein membrane that surrounds the 
embryo or blastocyst is weakened to facilitate 
hatching and the eventual implantation of the 
embryo.
Source: www.fertilitysmarts.com
Preimplantation genetic 
testing (PGT)
• A few cells surrounding the embryo are taken 
out and screened for genetic abnormalities and 
frozen and wait for the results on the 
appropriateness for transfer.
Source: www.fertilitysmarts.com
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