Real Time PCR is a portable unit comes with sample capacity of 16×0.2 ml (2×8 well, double block), offers emission wavelength ranges from F1: 520 to 540 nm and factory calibrated dyes of F1: FAM/SYBR Green l. Adopted with American Marlow customized Peltier module, single channel and double 8-wells blocks design with analysis software that can run two different design at a same time. Built in with high sensitivity photoelectric detector, 7 inch color TFT touch screen display of 1280×800 pixels with win 10 operating system and long life LED excitation. Equipped with powerful software analysis function such as, genotyping, melting curve, absolute and relative quantification.