Uploaded on Feb 15, 2021
Polymerase Chain Reaction (PCR) is a commonly used laboratory technique used to detect the presence of a particular DNA by multiplying it to make it easily detectable. PCR testing is used in different areas of biology. A PCR test has over the years been used in different fields. Below are some applications of a PCR test. For more information on the applications of a PCR test, visit our website at https://www.harleymedic.co.uk/
APPLICATIONS OF A PCR TEST.
APPLICATIONS OF A PCR
TEST.
APPLICATIONS OF A PCR TEST.
Polymerase Chain Reaction (PCR) is a commonly used laboratory technique used to detect the
presence of a particular DNA by multiplying it to make it easily detectable. PCR testing is used
in different areas of biology. A PCR test has over the years been used in different fields. Below
are some applications of a PCR test.
The first application of PCR test is in the field of forensic and applied sciences. Here PCR tests
are used in criminal investigations to identify the DNA that was left behind. It helps identify the
victim or even come up with a suspect. It relies on the amplification of individuals’ unique
short Tandem repeats (STRs) on gene DNA to help differentiate individuals
The second application of PCR tests is in agriculture. The most common use in the field is to
identify the presence or absence of genetically modified material in a product. It is further used
to identify the presence of a food pathogen and plant genotypes for breeding.
APPLICATIONS OF A PCR TEST.
Another application for PCR test is it is used to detect the presence of bacterium or DNA virus in an
individual. By taking a sample and exposing it to different conditions to ensure in the presence of
the DNA in question it will multiply hence easily detectable. PCR tests are more sensitive out of the
existing methods used to detect pathogens. Due to the issue of Coronavirus, PCR tests have become
more common. Here PCR tests involve three very important steps, the first step is the Denaturation
stage. Extraction of DNA from specimen occurs. This could be inform of a swab sample taken from
the throat and nose. Once the DNA is obtained, amplification of the said DNA is carried out. Where
separation of the two strands of DNA double helix is separated by being exposed to different
conditions in this case raising the temperatures. The second step is the annealing step and finally
extension where the multiplication of the DNA antigen occurs making it easily detectable.
For more information on the applications of a PCR test, visit our website at
https://www.harleymedic.co.uk/
APPLICATIONS OF A PCR
TEST.
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