Iroatechnologies1141
Uploaded on Jan 31, 2024
In the field of metabolomics, there are three key techniques that are commonly used to analyze and study metabolites. These techniques, known as iroatech, have revolutionized the way researchers approach metabolomics research. The first technique within iroatech is Ionization. This involves the conversion of analytes into ions, which allows for their detection and measurement. Different ionization methods such as electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) are employed depending on the nature of the samples being analyzed. The second technique is Retention Time Analysis. This involves separating metabolites based on their retention times in a chromatographic column. Gas chromatography (GC) and liquid chromatography (LC) are commonly used in this process to achieve high-resolution separation. Visit Us:- https://www.iroatech.com/metabolomics-analysis-and-iroa-quantitation/
What Are the 3 Techniques Used in Metabolomics?
Introduction to
Metabolomics
Metabolomics is the study of small molecules, commonly known as
metabolites, within cells, biofluids, tissues, or organisms. It provides
valuable insights into the biochemical processes associated with
various cellular activities.
Technique 1: Mass Spectrometry
Principle of Mass Spectrometry Applications in Metabolomics
Mass spectrometry is a powerful analytical Mass spectrometry is widely used in
technique used to identify the compounds metabolomics for biomarker discovery,
present in a sample based on the mass-to- studying drug metabolism, and
charge ratio of ions. It helps in understanding disease mechanisms at a
characterizing metabolites and molecular level.
understanding metabolic pathways.
Technique 2: Nuclear Magnetic
Resonance (NMR)
Principle of NMR Applications in Metabolomics
NMR spectroscopy is based on the NMR is used for identifying, profiling, and
interaction of magnetic fields with atomic quantifying metabolites, especially in
nuclei. It provides information about the complex biological samples. It plays a
molecular structure and allows non-invasive crucial role in metabolic phenotyping and
and quantitative analysis of metabolites. disease diagnostics.
Technique 3: Gas Chromatography-
Mass Spectrometry (GC-MS)
Principle of GC-MS Applications in Metabolomics
GC-MS separates and analyzes compounds GC-MS is applied to investigate metabolic
based on their boiling points and molecular pathways, identify biomarkers, and explore
characteristics. It's highly sensitive and can the impact of environmental and genetic
identify and quantify a wide range of factors on metabolic profiles in biological
metabolites in biological samples. systems.
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