Macro-Centrifuge-Tubes-Types-Benefits-and-Best-Practices
                     Macro Centrifuge Tubes: 
Types, Benefits, and Best 
Practices
Macro centrifuge tubes are indispensable in modern labs. They 
enable efficient processing of large sample volumes. This 
presentation explores their types, benefits, and usage.
Types of Macro Centrifuge Tubes
Polypropylene (PP) Polycarbonate (PC)
Common, autoclavable, cost-effective with good High clarity, good impact strength, but not autoclavable.
chemical resistance.
Polyallomer (PA) PETG
Combines PP and PE properties, high strength, and Shatter-resistant, good for frozen storage down to -80°C.
autoclavable.
Key Features & Specifications
Material Composition Volume Graduation
Density, melting point, and chemical compatibility Accuracy +/- 2%, molded or printed volume markings.
are important.
Maximum RCF Rating Sealing Mechanism
5,000xg to 20,000xg depending on material and design. Leak-proof screw cap, snap cap, or plug seal are critical.
Benefits of Using Macro Centrifuge Tubes
Large Capacity Scalability Cost-Effective Versatility
Macro centrifuge tubes offer large capacity and scalability. They are cost-effective, and compatible with centrifuges.
Applications in Cell Culture
Cell Harvesting
Efficiently collect cells from culture media.
Media Exchange
Quickly remove spent media.
Cell Lysate Preparation
Prepare lysates for extraction.
Example: Harvesting CHO cells from a 10L bioreactor is now simple.
Protein Purification and Nucleic Acid Extraction
Protein Precipitation Nucleic Acid Isolation
Concentrate proteins with ammonium sulfate precipitationIs.olate DNA and RNA from samples.
Processing 500mL of bacterial culture for plasmid DNA is now simple. Also used for collecting fractions from 
chromatography columns.
Best Practices for Using Macro Centrifuge Tubes
Balance the Rotor
1 Prevent centrifuge damage.
Temperature Considerations
2 Adhere to limits.
Chemical Compatibility
3 Verify compatibility.
Ensure caps are tightened to prevent leakage. Follow autoclaving procedures properly to avoid mishaps.
Conclusion: Maximizing Efficiency and Reliability
Macro centrifuge tubes are essential for large sample volumes. Selecting the right tube ensures reliable results.
Proper usage minimizes risks of sample loss and centrifuge damage. They improve efficiency of your experiments.