THE BENEFITS OF AUTOMATINGNGS LIBRARY PREP
                     THE BENEFITS OF AUTOMATING 
NGS LIBRARY PREP
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Increased use of next-generation sequencing (NGS) 
has made it a useful tool in the diagnostic journey of 
many patients. However, many labs are forced to 
depend on send-out services for NGS testing since the 
conversion of native DNA to a barcoded library 
detectable by NGS is a highly technical procedure that 
requires substantial time and chemicals and is prone 
to mistakes. 
Time and money may be saved, as well as errors avoided, by 
using specialized automation solutions for preparing NGS 
Library preparation, such as liquid handling equipment and 
integrated microfluidic systems. When all together, the 
advantages of automating NGS library creation in a clinical 
laboratory setting are substantial.
When doing next-generation sequencing, what goes into 
making the library?
There are two primary steps leading up to NGS sequencing, and 
both may be automated. First is sample preparation, which 
entails separating DNA from the collected tissue (e.g., blood, 
saliva) and subjecting it to chemical or mechanical disruption to 
release the DNA from the nucleus. The second phase, library 
preparation, entails a great deal of tedium and nuance. In order 
to get your library ready for short-read sequencing, you’ll need 
to do the following:-
• The first step is to fragment the DNA into manageable 
chunks that can be read by a sequencer.
• Attach the DNA fragments to sample-specific adapters.
• Attach the DNA-adapter hybrids to a solid state for 
amplification and sequencing
Time- and money-saver
The tedious and monotonous process of preparing NGS 
libraries is often regarded as the bottleneck of DNA 
sequencing. Clinical genetics labs waste time and money 
when they use a manual procedure since it involves more 
effort to prepare reagents is repetitious and is prone to 
mistakes. 
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