The increased utilization of next-generation sequencing (NGS) has transformed it into a valuable tool in the diagnostic process for numerous patients. However, many laboratories currently rely on external services for NGS testing due to the complex and error-prone nature of converting native DNA into a barcoded library that can be detected by NGS. Fortunately, specialized automation solutions, such as liquid handling equipment and integrated microfluidic systems, offer the potential to save time, money, and minimize errors in NGS library preparation within clinical laboratory settings.